Green Stone Swiss Co ., ltd.

SIRT6 Direct Fluorescent Assay Kit

Stability : 6 months
Storage : -80°C
Description :
SIRT6 is a nuclear protein that has both ADP-ribosyl transferase and deacetylase activities. SIRT6 associates specifically with telomeres and functions at chromatin to decrease NF-κB signaling.  SIRT6 Direct Fluorescent Screening Assay provides a convenient fluorescence-based method for screening SIRT6 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Arg-His-Lys-Lys(ε-acetyl)-AMC, is incubated with human recombinant SIRT6 along with NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product having excitation and emission at 350-360 nm and 450-465 nm, respectively.

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