Protocatechuate Dioxygenase

Stability : 6 months
Formulation : 20 mM potassium phosphate, pH 7.2, containing 100 mM sodium chloride
Description :
Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2. The PCD holoenzyme consists of α (23 kDa) and β (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.2 Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex. PCD is an important component of bacterial metabolism of aromatic compounds found in the soil.
Purity : ≥90%
Storage : -80°C