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Reagents
5-Lipoxygenase (potato)
Formulation : A solution in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfateStability : 6 monthsDescription : It was isolated from fresh potato tubers · One unit of enzyme consumes one nmol of oxygen per minute at 25°C in 0.1 M phosphate buffer, pH 6.3, containing 200 µM linoleate
Caspase-8 (human recombinant)
Formulation : A solution in 20 mM Tris-HCl, pH 8.0, containing 500 mM sodium chloride, 20 mM .beta.-mercaptoethanol, 2.5 mM EDTA, 10% sucrose, and 0.1% CHAPSStability : 6 monthsDescription : Caspase-8 is a member of the interleukin-1 converting enzyme (ICE) family of cysteine proteases. It exists
15-Lipoxygenase-2 (human recombinant)
Formulation : PBS, pH 7.4, 1 mM DTT and 20% glycerolStability : 6 monthsDescription : Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1. Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea
15-Lipoxygenase (soybean P1)
Formulation : A solution in 0.1 M Tris-HCl, pH 7.0Stability : 1 yearDescription : Lipoxygenase-catalysed arachidonic acid metabolism plays a key role in colorectal carcinogenesis and has the potential to be modulated by phenolic compounds. Plant-based foods are rich sources of phenolic compounds
15-Lipoxygenase (soybean P1) - Purified
Formulation : A solution in 0.1 M Tris-HCl buffer, pH 7.0, containing 10% glycerolStability : 1 year
12-Lipoxygenase (porcine leukocyte)
Molecular Formula : MonomerStability : 1 yearFormulation : A solution in 20 mM phosphate buffer, pH 7.5Description : 12-Lipoxygenase was purified from porcine leukocytes as described (12). Purified enzyme (0.25 mg) was digested with Achromobacterproteinase I (3 ,g) in 170 ,ul of 0.1 M Tris HCl(pH 8